Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1198508 | Journal of Chromatography A | 2016 | 13 Pages |
•Improved reaction conditions for benzoyl chloride labeling for HPLC–MS/MS analysis.•Novel assay of 70 neurologically relevant compounds using benzoyl chloride labeling.•Analysis of rat dialysate, fly tissue homogenate and hemolymph, human CSF and serum.•Stable-isotope labeled internal standard for all analytes for quantification.
Widely targeted metabolomic assays are useful because they provide quantitative data on large groups of related compounds. We report a high performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) method that utilizes benzoyl chloride labeling for 70 neurologically relevant compounds, including catecholamines, indoleamines, amino acids, polyamines, trace amines, antioxidants, energy compounds, and their metabolites. The method includes neurotransmitters and metabolites found in both vertebrates and insects. This method was applied to analyze microdialysate from rats, human cerebrospinal fluid, human serum, fly tissue homogenate, and fly hemolymph, demonstrating its broad versatility for multiple physiological contexts and model systems. Limits of detection for most assayed compounds were below 10 nM, relative standard deviations were below 10%, and carryover was less than 5% for 70 compounds separated in 20 min, with a total analysis time of 33 min. This broadly applicable method provides robust monitoring of multiple analytes, utilizes small sample sizes, and can be applied to diverse matrices. The assay will be of value for evaluating normal physiological changes in metabolism in neurochemical systems. The results demonstrate the utility of benzoyl chloride labeling with HPLC–MS/MS for widely targeted metabolomics assays.