Article ID Journal Published Year Pages File Type
1198826 Journal of Chromatography A 2016 8 Pages PDF
Abstract

•A flow-modulated GC × GC–MS method has been developed for perfume allergen analysis.•MS-compatible gas flows were used.•The 54 allergens indicated by the SCCS were subjected to GC × GC–MS determination.•Allergen quantification was performed by using extracted-ions.

The present research is based on the development and use of a flow-modulation (FM) comprehensive two-dimensional gas chromatography–quadrupole mass spectrometry (GC × GC–qMS) method for the determination of recently-highlighted (by the Scientific Committee on Consumer Safety) fragrance allergens (54) in cosmetics. FM GC × GC–qMS conditions were finely tuned to generate flow conditions (≈7 mL min−1) compatible with the qMS system used. Six-point calibration curves, over the range 1, 5, 10, 20, 50, 100 mg L−1, were constructed for the 54 target allergens, with satisfactory linearity observed in all cases. Absolute quantification was performed by using extracted ions; target analyte identification was performed through measurement of ion ratios (qualifier/quantifier), full-scan MS database matching and the use of linear retention indices. Additional analytical figures of merit subjected to measurement were intra-day repeatability, accuracy at the 25 and 5 mg L−1 levels, and limits of detection and quantification. The number of data points per peak, along with mass spectral skewing, was also subjected to evaluation. Finally, the FM GC × GC–qMS method was used not only for the quantification of target allergens in five commercial perfumes, but also for general qualitative profiling.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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