Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1198999 | Journal of Chromatography A | 2015 | 6 Pages |
•Biocompatible chiral monolithic stationary phase was prepared by ATRP methods.•The monolithic stationary phase can be used as chiral restricted access material.•Enantiomers in biological sample were detected by HPLC with direct injection.
Novel biocompatible chiral monolithic stationary phase was prepared by reverse and direct atom transfer radical polymerization (ATRP) methods. By taking advantages of the controlled/living property of ATRP method, the chiral monolith was prepared by reverse ATRP (RATRP) firstly. An attractive feature of RATRP is the prepared polymer containing a terminal radically transferable atom that can initiate another post-polymerization reaction by direct ATRP. Then, the biocompatible poly(hydroxyethyl methacrylate) (PHEMA) was grafted on the surface of the chiral monolith by direct ATRP as a diffusion barrier for proteins. This biocompatible chiral monolith was successfully used as restricted access stationary phase for determination of enantiomers in biological samples with direct injection by high-performance liquid chromatography (HPLC).