A straightforward methodology for designing continuous monoclonal antibody capture multi-column chromatography processes

•Simplify process development for multi column chromatography.•Minimal requirements: just a few single column experiments.•Provides understanding of the complete experimental space.•Validated method through a series of multi-column chromatography processes.•Lowers the barrier to adoption of continuous bioprocessing.

A simple process development strategy for continuous capture multi-column chromatography (MCC) is described. The approach involves a few single column breakthrough experiments, based on several simplifying observations that enable users to rapidly convert batch processes into well-designed multi-column processes. The method was validated using a BioSMB® (Pall Life Sciences) lab scale multi-column system and a mAb capture process employing Protein A resin. The approach enables users to optimize MCC processes based on their internal preferences and constraints without requiring any mathematical modeling expertise.