Evaluation of perphenylcarbamated cyclodextrin clicked chiral stationary phase for enantioseparations in reversed phase high performance liquid chromatography

•The column characterization of perphenylcarbamated cyclodextrin (CD) clicked chiral stationary phase (CSP).•The enantioseparation 26 recemates with the CD clicked CSP in reversed phase high-performance liquid chromatography.•The detailed correlation study of flow rate, column temperature, organic modifier and buffer on the enantioselectivity of the clicked CSP.•The excellent chiral resolutions readily achieved with the high column efficiency CSP.

In this study, perphenylcarbamated cyclodextrin clicked chiral stationary phase (CSP) was developed with high column efficiency. The characteristics of the column were evaluated in terms of linearity, limit of detection and limit of quantification. The enantioselectivity of the as-prepared clicked CSP was explored with 26 recemates including aryl alcohols, flavanoids and adrenergic drugs in reversed phase high-performance liquid chromatography. The effect of separation parameters including flow rate, column temperature, organic modifier and buffer on the enantioselectivity of the clicked CSP was investigated in detail. The correlation study of the analytes structure and their chiral resolution revealed the great influence of analytes’ structure on the enantioseparations with cyclodextrin CSP. Methanol with 1% of triethylammonium acetate buffer (pH 4) was proved to be the best choice for the chiral separation of basic enantiomers.