Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1199256 | Journal of Chromatography A | 2015 | 8 Pages |
Abstract
This paper aims to demonstrate the effect of pressure variations in modifying analyte retention behavior in SFC. There is a general understanding that in SFC increasing pressure decreases the retention factor (kâ²), and vice versa. What is not clearly discussed or explained in any recent literature is that these variations can be very different at different operating pressures, temperatures and modifier concentrations. It is important to have a clearer understanding on these variabilities during method development and results analysis. In this paper the nature of kâ² variation with pressure, at different temperatures and modifier concentrations, will be explained with the help of isopycnic plots of CO2 and CO2Â +Â methanol mixtures.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Abhijit Tarafder, Jason F. Hill, Pamela C. Iraneta, Kenneth J. Fountain,