Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1199918 | Journal of Chromatography A | 2013 | 6 Pages |
•A new adsorbent for removing endotoxins from proteins solutions is described.•Solid allantoin removes >99.9% endotoxins in <5 min.•Endotoxin binding on allantoin crystals appears to be mediated by hydrogen-bonding.•The method is more effective and faster than other methods for endotoxin clearance.
In this study we present a simple and robust method for removing endotoxins from protein solutions by using crystals of the small-molecule compound 2,5-dioxo-4-imidazolidinyl urea (allantoin) as a solid phase adsorbent. Allantoin crystalline powder is added to a protein solution at supersaturated concentrations, endotoxins bind and undissolved allantoin crystals with bound endotoxins are removed by filtration or centrifugation. This method removes an average of 99.98% endotoxin for 20 test proteins. The average protein recovery is ∼80%. Endotoxin binding is largely independent of pH, conductivity, reducing agent and various organic solvents. This is consistent with a hydrogen-bond based binding mechanism. Allantoin does not affect protein activity and stability, and the use of allantoin as a solid phase adsorbent provides better endotoxin removal than anion exchange, polymixin affinity and biological affinity methods for endotoxin clearance.