| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1200077 | Journal of Chromatography A | 2014 | 8 Pages |
•In-house validation of a pre-column derivatization using AQC reagent to determine amino acids in gelatin.•The carrageenan was recommended to be used as blank assay for this analytical measurement.•Only one submission LOQ representing overall amino acids (37.5 pmol/μl) for routine analysis.•The linearity for overall amino acids was assessed in detail with r2 more than 0.9.•The findings data were reproducible and the method is fit for routine analysis.
In-house method validation was conducted to determine amino acid composition in gelatin by a pre-column derivatization procedure with the 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate reagent. The analytical parameters revealed that the validated method was capable of selectively performing a good chromatographic separation for 18 amino acids in less than 40 min; the overall detection and quantitation limit for amino acids fell into ranges of 5.68–12.48 and 36.0–39.0 pmol/μl, respectively; the matrix effect was not observed, and the linearity range was 37.5–1000 pmol/μl. The accuracy (precision and recovery) analyses of the method were conducted under repeatable conditions on different days in random order. Method precision revealed by HorRat values was significantly less than 2, except for histidine with a precision of 2.19, and the method recoveries had a range of 80–115% except for alanine which was recovered at 79.4%. The findings were reproducible and accurately defined, and the method was found to be suited to routine analysis of amino acid composition in gelatin-based ingredients.
