A new stationary phase for high performance liquid chromatography: Calix[4]arene derivatized chitosan bonded silica gel

•New calix[4]arene derivatized chitosan bonded stationary phase (CCS4) was prepared.•CCS4 provided the multiple interactions with polar and non-polar solutes.•CCS4 could perform both RPLC and HILIC separations.

In the present paper, a new calix[4]arene derivatized chitosan bonded stationary phase (CCS4) for high performance liquid chromatography (HPLC) was synthesized and characterized. Its chromatographic performance and retention mechanism were evaluated in reversed-phase mode compared with ODS using different solute probes including mono-substituted benzenes, phenols and nucleosides. The results showed that CCS4 stationary phase could provide various interactions with solutes, such as hydrophobic, hydrophilic, π–π, and inclusion interactions. It could perform the mixed-mode separation including RP and HILIC.