Facile determination of the absolute configurations of α-hydroxy acids by chiral derivatization coupled with liquid chromatography–mass spectrometry analysis

α-Hydroxy acids are commonly encountered in natural bioactive molecules. For convenient determination of the absolute configurations of α-hydroxy acids, a simple method, named the “O-Marfey method” was developed using a chiral derivatization strategy with (1-fluoro-2,4-dinitrophenyl-5)-l-alanine amide (l-FDAA). The liquid chromatography–mass spectrometry (LC–MS) analysis of the derivatives clearly determined the absolute configurations of various α-hydroxy acids based on their elution times and sequences. This new method is operationally simple with short reaction time and sensitive enough for application at a sub-milligram scale without any purification. This method also enables the simultaneous chirality analysis of α-hydroxy acids and α-amino acids. The absolute configuration of a natural depsipeptide bearing an α-hydroxy acid and α-amino acids was successfully determined by our O-Marfey application.

► Facile method for the absolute configuration of α-hydroxy acids by LC–MS analysis. ► Simultaneous chirality analysis of α-hydroxy acids and α-amino acids. ► The reliability was confirmed by determining the chirality of a natural compound.