| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1201599 | Journal of Chromatography A | 2013 | 6 Pages |
Halo C18 column (fused core particles) and Chromolith RP18 column (monolith) were evaluated in liquid chromatography in order to analyze methylated-β-cyclodextrins (Me-β-CD) with various degrees of substitution, DS such as the number of methyl groups per cyclodextrin ring. Chromolith RP18 enables a performing analysis of Me-β-CD with low DS but is not suitable for dimethyl-β-cyclodextrins (DM-β-CD). On the other hand, Halo C18 column allows an improved fingerprint of CDs having a DS from 4.9 up to a value major than 14 and avoiding the use of various chromatographic systems. Thus, liquid chromatography performed with this column and an evaporative light scattering detector can be used as a generic system for methylated CD analysis. Moreover, fused core particles of Halo C18 column enables a rapid analysis and liquid chromatography coupled with electrospray-mass spectrometry appears as a powerful tool to determine co-elution and to characterize various isomers of complex methylated-β-cyclodextrin mixtures.
► Halo C18 and Chromolith RP18 columns were evaluated to analyze methylated CDs by LC. ► Halo C18 column is suitable for rapid LC analysis of various CDs. ► LC-ELSD with this column was proposed as a generic system for CD analysis.
