Complementary enantiorecognition patterns and specific method optimization aspects on immobilized polysaccharide-derived chiral stationary phases

The immobilized polysaccharide-derived chiral stationary phases (CSPs) combine the benefits of broad application scope and high preparative potential with CSP robustness and universal solvent compatibility. Strategies for efficient and straightforward method development with these CSPs were previously overviewed. In the current study, six CSPs with different structural features in the immobilized series were examined for their complementary properties in separation of enantiomers. Some method development aspects related to polysaccharide-derived CSPs in general and certain specific features observed on these immobilized supports, such as the effects of mobile phase, sample solvent and mobile phase additive on chiral separation, are also discussed.

► Six immobilized polysaccharide-derived CSPs are complementary in chiral separation. ► High success rate is achieved with only two different mobile phase systems. ► Mobile phase can impact the thermodynamic and kinetic aspects. ► The sample solvent should be considered in case of peak deformation. ► Mobile phase additive may play a major role for chiral separation.