Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1202201 | Journal of Chromatography A | 2011 | 5 Pages |
Abstract
A novel fluorescent labeling method for alprenolol was developed based on Mizoroki-Heck coupling reaction. We designed and synthesized fluorescent aryl iodide, 4-(4,5-diphenyl-1H-imidazol-2-yl)iodobenzene (DIBI) as a labeling reagent. DIBI has a lophine skeleton carrying an iodide atom acting as fluorophore and reactive center, respectively. In order to evaluate the usefulness of DIBI, a high-performance liquid chromatography (HPLC) with fluorescence detection method was developed for the determination of alprenolol as a model compound of terminal double bond. The fluorescent labeling of alprenolol with DIBI was achieved in the presence of palladium acetate as a catalyst, and the labeled alprenolol was detected fluorometrically. In addition, it was found that the fluorescence of DIBI derivative increased and red shifted when compared with that of DIBI. Furthermore, the proposed method could be applied to determine the alprenolol concentration in rat plasma after administration of alprenolol without interferences from biological components. The detection limit (S/NÂ =Â 3) for alprenolol in rat plasma was 0.74Â ng/mL (30Â fmol on column).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Naoya Kishikawa, Mizuho Ohkuma, Mitsuhiro Wada, Kaname Ohyama, Rie Ikeda, Kenichiro Nakashima, Naotaka Kuroda,