Taylor dispersion analysis with two detection points on a commercial capillary electrophoresis apparatus

This work describes a simple technical modification for doing Taylor dispersion analysis with two UV detection points on a commercial capillary electrophoresis apparatus. So far, double UV detection was only possible using specific detectors that are external to the capillary electrophoresis apparatus. In this work, the detection interface of the capillary electrophoresis apparatus was easily modified to allow the introduction and the superposition of two capillary windows in the same interface (at the same detection point). This modification made possible the double detection of the sample zone in Taylor dispersion analysis using a loop. The peak dispersion using the modified interface was similar to that obtained on a non-modified UV interface. Diffusion coefficients (and the corresponding hydrodynamic radii) of small molecule and proteins were determined in good agreement with values of the literature and with RSD lower than 5%.

► Double UV detection on commercial CE apparatus without external detector. ► Simple technical modification on detection interface for double detection. ► Application to the sizing of protein and small molecule by Taylor dispersion analysis.