Article ID Journal Published Year Pages File Type
1204199 Journal of Chromatography A 2010 5 Pages PDF
Abstract

Tetrodotoxin (TTX), a toxic compound found in some puffers can cause death to humans through consumption. We have developed a simplified method for the screening of TTX in puffers using GC–MS. A puffer tissue of 0.5 g was treated with 5 mL of 0.1% acetic acid, followed by alkaline hydrolysis, LLE or liquid–liquid extraction and N-methyl-N-TMS-trifluoroacetamide derivatization. The developed method used only a small sample and solvent, simplified LLE and derivatization procedures and short chromatographic analysis (8.2 min). All of these contribute to cost-saving, enhanced sample throughput and high sensitivity of the screening assay. The developed method was validated and proved to be within the acceptable range.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , ,