Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1205210 | Journal of Chromatography A | 2008 | 4 Pages |
Abstract
This work demonstrates that cyclohexanedimethanol is an effective nonflammable organic solvent for the pilot scale reversed phase chromatography of recombinant apolipoprotein A-IMilano. Cyclohexanedimethanol has low viscosity in water, enabling the use of conventional low pressure process scale chromatography columns and hardware. Results from pilot scale manufacturing using a 30 cm diameter CG71M packed column indicate that a 5.7 log reduction in E. coli host cell protein with >80% yield can be obtained for the purification of apolipoprotein A-IMilano.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Alan K. Hunter, Sydney D. Hoeltzli, Gary V. Johnson, Mark E. Gustafson, Sa V. Ho,