Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1205906 | Journal of Chromatography A | 2009 | 11 Pages |
Abstract
Detergent removal from mixed micelles was combined with preparative size exclusion chromatography (SEC) on Sephacryl S 500 HR to prepare unilamellar and spherical liposomes of defined sizes between 50 and 100 nm with a very narrow size distribution (RSD of vesicle diameter between 13% and 25%). For neutral phosphatidylcholine and negatively charged phosphatidylcholine/phosphatidylglycerol liposome preparations, efficient sizing at the preparative scale was demonstrated by analyzing isolated SEC peak fractions with cryo-transmission electron microscopy and dynamic light scattering. The number-weighted average vesicle diameters obtained using both methods are in very good agreement for fractions of low polydispersity.
Keywords
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Martin Holzer, Sabine Barnert, Joachim Momm, Rolf Schubert,