| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1206242 | Journal of Chromatography A | 2008 | 8 Pages |
Abstract
A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF3 or BCl3 in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Pedro Araujo, Thu-Thao Nguyen, Livar Frøyland, Jingdong Wang, Jing X. Kang,