Magnetic solid-phase extraction based on diphenyl functionalization of Fe3O4 magnetic nanoparticles for the determination of polycyclic aromatic hydrocarbons in urine samples

Superparamagnetic Fe3O4 diphenyl nanoparticles were prepared according to a solvothernal procedure and characterized by X-ray diffraction, infrared spectroscopy, surface area measurements, scanning electron microscopy, X-ray photoelectron spectroscopy and transmission electron microscopy. The magnetic phases present in the nanoparticle samples were analyzed by thermomagnetic analysis and the samples’ magnetic properties were studied by vibrating sample magnetometry. The resulting nanoparticles having an average diameter of 200 nm were then used as solid-phase extraction sorbent for the determination of polycyclic aromatic hydrocarbons in urine samples. Method validation proved the feasibility of the developed beads for the quantitation of the investigated analytes at trace levels obtaining lower limit of quantitation values in the ng/l range. A good precision with coefficients of variations always lower than 15% was obtained. Finally, the superior extraction performance of the synthesized nanoparticles with respect to commercially available beads was proved.

► Synthesis of superparamagnetic nanoparticles. ► Functionalization with a diethoxydiphenylsilane coating. ► Analysis of PAHs in urinary samples of smoking and no-smoking people.