Article ID Journal Published Year Pages File Type
1206542 Journal of Chromatography A 2008 12 Pages PDF
Abstract

Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug–plug EMMA, improving sensitivity by extending enzyme–substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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