Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1206641 | Journal of Chromatography A | 2009 | 8 Pages |
Abstract
A completely automated procedure for the purification and desalting of proteins with a polyhistidine purification tag prior to mass spectrometry analysis is presented. The system is ideal for rapid quality control and optimization studies and it provides researchers with a straightforward, reliable tool for studies of recombinant proteins. Forty-eight samples can be prepared within 4.5Â h and only small cultivation and buffer volumes are needed. In this proof of concept, 19,000-35,000Â Da recombinant proteins from both crude and clarified cell lysates were successfully prepared for subsequent analysis by electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry as well as by gel electrophoresis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Johanna Steen, Margareta Ramström, Mathias Uhlén, Sophia Hober, Jenny Ottosson,