Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1206646 | Journal of Chromatography A | 2009 | 5 Pages |
Abstract
Capillary electrophoresis (CE) was compared with reversed-phase liquid chromatography for its ability to separate native and deamidated peptides. CE is shown to provide superior resolution of these peptides due to its charge-based separation mechanism. Fraction collection performed using a standard CE instrument equipped with a 96-well plate permits subsequent characterization by nanospray mass spectrometric (MS) analysis. Additional in-depth analysis by MS/MS is able to provide the location of the deamidation site based on y-ion mass shifts of 1 Da.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lynn A. Gennaro, Oscar Salas-Solano,