Development of C18-functionalized magnetic silica nanoparticles as sample preparation technique for the determination of ergosterol in cigarettes by microwave-assisted derivatization and gas chromatography/mass spectrometry

Ergosterol is one of the important precursors of tumorigenic polynuclear aromatic hydrocarbons. A large amount of ergosterol is present in mildewy cigarettes, which derives from fungal contaminations. In this paper, a novel approach based on C18-functionalized magnetic silica nanoparticles (C18-f-MS NPs) coupled with microwave-assisted derivatization and gas chromatography/mass spectrometry (GC/MS) was developed for the rapid enrichment and determination of ergosterol in cigarettes. Due to that, microwave-assisted derivatization requires very short time (several minutes), and the extraction and concentration of ergosterol become the key step in the sample preparation process. In this study, the prepared C18-f-MS NPs with its unique properties (high surface area and strong magnetism) provided an efficient way for extraction and concentration of ergosterol in the samples. Additionally, the analyte of ergosterol adsorbed with C18-f-MS NPs in cigarettes can be simply and rapidly isolated (only about 2 s) through placing a strong magnet on the bottom of container. In this work, different parameters such as added amounts of C18-f-MS NPs, extraction temperature, and extraction time were optimized to enhance the extraction efficiency. Method validations (linear range, detection limit, precision, and recovery) were also studied. The results obtained by the optimal conditions showed that the proposed method based on C18-f-MS NPs was a simple, high efficient, and had a rapid approach for the enrichment of ergosterol in cigarettes and was successfully applied to the analysis of ergosterol in normal and mildewy cigarettes followed by microwave-assisted derivatization and GC/MS.