| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1206773 | Journal of Chromatography A | 2009 | 7 Pages |
Abstract
A gradient LC method for the analysis of capreomycin sulfate and its related substances was developed. The chromatographic conditions include the use of a Hypersil base deactivated C18 (250 mm × 4.6 mm, 5 μm) column maintained at 25 °C, a mobile phase containing acetonitrile, phosphate buffer pH 2.3 and 0.025 M hexanesulfonate at a flow rate of 1.0 mL/min and UV detection performed at 268 nm. Good separation of the four active components of capreomycin and eleven unknown impurities was achieved. A system suitability test to check the quality of the separation is specified. The method shows good repeatability, linearity and robustness.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Swapna Mallampati, Shan Huang, Dunge Ashenafi, Elise Van Hemelrijck, Jos Hoogmartens, Erwin Adams,