Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1207099 | Journal of Chromatography A | 2009 | 6 Pages |
Abstract
An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jeremy Lee, Anne Tscheliessnig, Allen Chen, Yih Yean Lee, Gloria Adduci, Andre Choo, Alois Jungbauer,