Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1207309 | Journal of Chromatography A | 2008 | 4 Pages |
Abstract
We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Daniele Oliva, Sabina Di Maio, Giorgia Sisino, Daniele Bellavia, Rainer Barbieri,