| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1208233 | Journal of Chromatography A | 2007 | 6 Pages |
Abstract
In this paper, an analysis of the influence of the salt types, NaCl, NaCH3COO, Na2SO4 and Na3C6H5O7, on the isocratic retention behaviour of bovine serum albumin (BSA) on two anion-exchangers media (Source 30Q and TSK Gel Super Q 5 PW) has been presented. The retention data demonstrated that the mechanism of protein retention in ion-exchange chromatography (IEC) involves interactions between the protein solute, the mobile phase constituents and the stationary phase. The effect of protein activity coefficient in the mobile phase on the protein retention volumes is verified.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sattar Al-Jibbouri,