Article ID Journal Published Year Pages File Type
1208273 Journal of Chromatography A 2007 9 Pages PDF
Abstract

Warfarin is a widely used oral anticoagulant which is mostly administrated as a racemic mixture containing equal amount of R- and S-enantiomers. The two enantiomers are shown to exhibit significant differences in pharmacokinetics and pharmacodynamics. In this study, a new chiral micellar electrokinetic chromatography–mass spectrometry (MEKC–MS) method has been developed using a polymeric chiral surfactant, polysodium N-undecenoyl-l,l-leucyl-valinate (poly-l,l-SULV), as a pseudostationary phase for the chiral separation of (±)-warfarin (WAR) and (±)-coumachlor (COU, internal standard). Under optimum MEKC–MS conditions, the enantio-separation of both (±)-WAR and (±)-COU was achieved within 23 min. Calibration curves were linear (R = 0.995 for (R)-WAR and R = 0.989 for (S)-WAR) over the concentration range 0.25–5.0 μg/mL. The MS detection was found to be superior over the commonly used UV detection in terms of selectivity and sensitivity with LOD as low as 0.1 μg/mL in human plasma. The method was successfully applied to determine WAR enantiomeric ratio in patients’ plasma undergoing warfarin therapy.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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