| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1208326 | Journal of Chromatography A | 2008 | 8 Pages |
Abstract
For the first time, the application of a commercial Shimadzu microchip electrophoresis system MCE-2010 equipped with an imaging UV detector for isoelectric focusing (IEF) of therapeutic proteins is reported. By proper adjustment of the pH gradient, samples with pI values ranging from 2.85 to 10.3 can be focused to the imaged part of the separation channel. Three therapeutic proteins (hirudin, erythropoietin, and bevacizumab) have been successfully focused on the microchip, and the results have been compared to conventional capillary IEF in terms of peak profile, pI values, and reproducibility.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Markéta Vlčková, Franka Kalman, Maria A. Schwarz,