Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1208351 | Journal of Chromatography A | 2006 | 7 Pages |
Abstract
For quantification of 3R,3â²R-zeaxanthin in plant derived food, a new diastereomeric dilution assay (DIDA) was developed using chiral high-performance liquid chromatography coupled to diode-array detection (HPLC/DAD). As diastereomeric internal standard (I.S.), 3R,3â²S-(meso)-zeaxanthin was applied. DIDA showed sufficient sensitivity with low limits of detection and quantification (18 μg 100 gâ1 and 45 μg 100 gâ1), a good inter-assay coefficient of variation (2.4%), determined by analysis of a certified BCR reference material, and a good recovery (94.7%). To prove the applicability of DIDA, various plant materials were anaylzed, showing that DIDA is highly advisable if saponification is applied during work-up to hydrolyze xanthophyll esters.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jörg Schlatterer, Svenja Maurer, Dietmar E. Breithaupt,