Article ID Journal Published Year Pages File Type
1208588 Journal of Chromatography A 2007 10 Pages PDF
Abstract

A rapid and sensitive high-performance liquid chromatography (HPLC) method with coulometric electrochemical and ultraviolet detections for analysis of vitamin B (B1, B6 and B12) in animal and plant foods has been developed. A combination of acid digestion and enzymatic extraction to release protein bound and phosphorylated vitamins followed by HPLC analysis was applied. The analyses were carried out on a LC18 column 5 μm (25 cm × 4.6 mm), using the mobile phase consisting of methanol–phosphate buffer (10:90) and 0.018 M trimethylamine, pH 3.55, following at 1.0 ml min−1. The method offers excellent linearity with regression coefficient r > 0.998, good repeatability and reproducibility and relatively short analysis time (17 min). The relative standard deviation (RSD) of the intermediate precision was satisfactory for all the vitamins studied and amounted to <7.3%. The repeatability of the method was <4.7%. The limits of quantification (LOQ) for pyridoxamine, pyridoxal, pyridoxine, vitamins B1 and B12 in seafood products were as follows: 2.1, 2.01, 0.99, 62.0 and 0.11 ng ml−1, respectively. The mean recovery values from a food products spiked with all five vitamins ranged from 92.3 to 101.3%, with a relative standard deviation less than 3.4%. The proposed separation and detection procedures were successfully applied for the simultaneous determination of vitamins B1, B6 and B12 in fruit juices and vitamins B6 and B12 in seafood.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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