| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1208630 | Journal of Chromatography A | 2008 | 8 Pages |
Abstract
Pre-packed MediaScout® MiniChrom columns of 2.5, 5 and 10 mL were investigated for screening three mixed-mode chromatography sorbents (HEA, PPA and MEP HyperCel™). Packing performance was of good quality and the three sorbents displayed higher capacity than traditional HIC sorbents in physiological-like conditions. Each sorbent offered a unique selectivity. Bovine β-lactoglobulin was partially purified after loading milk whey directly on HEA HyperCel sorbent. The combination of small pre-packed columns and SELDI-MS appeared to be a valuable strategy for high-throughput screening of chromatography sorbents and for enabling rapid process development and optimization.
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Authors
Virginie Brenac Brochier, Anthony Schapman, Patrick Santambien, Lothar Britsch,