| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1208892 | Journal of Chromatography A | 2007 | 7 Pages |
Abstract
We report a novel method for the extraction of α-1 antitrypsin (AAT) from plasma. This method uses an anion-exchange column and two metal chelate columns. The AAT is recovered in a 60% yield and a purity of over 90%. The AAT manufactured by this method was stable at 4 °C for 12 months without any stabilisers. Other proteins of therapeutic value and commercial interest are recoverable in good yields. The simplicity of this process makes it a suitable alternative to the traditionally employed ethanol precipitation method where high ethanol levels inactivate AAT.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Peter Kumpalume, Adrian Podmore, Christopher LePage, Joan Dalton,