| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1208909 | Journal of Chromatography A | 2006 | 5 Pages |
The determination of perfluorooctanoate (PFO) in articles of commerce has become increasingly important to understand if treated products are a possible source of PFO. An LC–MS/MS method for the determination of PFO in paper and textile using a dual labeled 13C-PFOA internal standard was successfully developed and validated. Residues of PFO were determined using an isocratic, reversed-phase high-performance liquid chromatography (HPLC) method with an ammonium acetate/methanol buffer. Ions monitored were 413 (parent) and 369 (daughter) for PFO and 415 (parent) and 370 (daughter) for dual labeled 13C-PFOA internal standard. As a precaution against ubiquitous PFO that occasionally occurs in mobile phase or instrument components, two Hypercarb™ cartridges (4 mm) were placed before the HPLC injector. Any PFO that was captured by the cartridges was removed before each injection by flushing the system with 100% methanol prior to equilibration with the isocratic mobile phase. Overall recovery and standard deviation over a 3 day validation regimen for samples (n = 54–55) fortified with PFOA at 5, 50, and 200 ng g−1 were 114 ± 4.9% for textile and 110 ± 7.6% for paper. The results also established a limit of detection (LOD) of 1 ng g−1 in textile and 2 ng g−1 in paper based upon S/N of the 5.0 ng g−1 fortification versus the untreated paper and textile.
