Article ID Journal Published Year Pages File Type
1209027 Journal of Chromatography A 2007 5 Pages PDF
Abstract

A simple method for the extraction of carotenoid pigments from frozen wet cells of red yeasts (Basidiomycota) and their analysis by reversed-phase HPLC using a C18 column and a water/acetone solvent system is described. Typical red yeast carotenoids belonging to an oxidative series from the monocyclic γ-carotene to 2-hydroxytorularhodin and from the bicyclic β-carotene to astaxanthin were separated. Pigment identity was confirmed by LC–atmospheric pressure chemical ionisation (APCI) mass spectrometry using similar chromatographic conditions.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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