Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1209042 | Journal of Chromatography A | 2007 | 5 Pages |
Abstract
A rapid, sensitive and selective method for the determination of tryptophan (Trp), serotonin (5-HT) and psychoactive tryptamines (PATs) by thin-layer chromatography (TLC) with fluorescence detection is proposed. These compounds form fluorophores on the developing plate by heating after spraying with sodium hypochlorite, hydrogen peroxide or potassium hexacyanoferrate(III)-sodium hydroxide reagent. Fluorescent spots (vivid blue) were observed by irradiation with ultraviolet light (365 nm). The detection limits of Trp, 5-HT and PATs were in the range from 0.01 μg to 0.06 μg. This method was effectively applied to the detection of confiscated PAT powder and PAT in abusers’ urine samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Noriyuki Kato, Takashi Kojima, Shinji Yoshiyagawa, Hikoto Ohta, Akira Toriba, Hideo Nishimura, Kazuichi Hayakawa,