| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1209265 | Journal of Chromatography A | 2007 | 6 Pages |
Abstract
A model is developed for correlating the adsorption of protein to hydroxyapatite in terms of pH, phosphate and imidazole. It provides a basis for manipulating the chromatography conditions so as to obtain the best possible fractionation. The adsorption mechanism is apparently a function of two calcium affinity interactions and cation exchange. We used immunoglobulins and a fusion protein with or without a poly(6)histidine tag for our investigation. The results are presented and discussed in the present study.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Paul K. Ng, Jie He, Pete Gagnon,