Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1209390 | Journal of Chromatography A | 2007 | 6 Pages |
Abstract
We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3 mM NaOH containing 1 mM NaOAc, and 200 mM NaOH was added for post-column reagent. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) for galactose were 25 ng/mL and 83 ng/mL, respectively. Linear dynamic range was from 4.67 mg/dL to 53.46 mg/dL (r2 = 0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ji-Seon Jeong, Hye-Ran Yoon, Seon-Pyo Hong,