Temperature as a variable in liquid chromatography: Development and application of a model for the separation of model drugs using water as the eluent

The use of high temperatures in liquid chromatography allows for the use of a purely aqueous mobile phase. At elevated temperatures water possesses many of the characteristics of organic solvents in terms of eluotropic strength, as well as having a lower viscosity. A model is developed, based on data obtained using a range of model drugs, which demonstrates the relationship between temperature, flow and pressure. Experimental data from different column types, at temperatures from 40 °C to 180 °C, is presented which matches well with the predicted data from the model.