| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1209606 | Journal of Chromatography A | 2006 | 5 Pages |
Abstract
An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Paul B. Myrdal, B. Steven Angersbach, Kelly Karlage, Philip J. Kuehl,