Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1209653 | Journal of Chromatography A | 2006 | 4 Pages |
Abstract
A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane–methanol–water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their 1H NMR and 13C NMR spectra.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Quan Bin Han, Jing Zheng Song, Chun Feng Qiao, Lina Wong, Hong Xi Xu,