Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1209784 | Journal of Chromatography A | 2006 | 7 Pages |
Abstract
A surfactant/polymer wall coating consisting of the doubly chained cationic surfactant dimethyldioctadecylammonium bromide (DODAB) and polyoxyethylene (POE) 40 stearate is investigated. The coating is formed by simply rinsing a capillary with a solution containing DODAB and POE 40 stearate. The resultant coating is semi-permanent-demonstrating stable electroosmotic flow (EOF) even after a 60 min high pressure rinse with buffer. The EOF (â0.45 ± (0.23) Ã 10â4 cm2 Vâ1 sâ1 at pH 7.4) is suppressed by more than a factor of ten compared to that observed for DODAB alone. Model protein mixtures were separated over a pH range of 3-10 with efficiencies of up to greater than 1 million plates/m for the basic proteins cytochrome c, lysozyme, ribonuclease A and α-lactalbumin, and the acidic proteins insulin chain A, trypsin inhibitor, and α-chymotrypsinogen A. Migration time reproducibility was 0.5-4.0% from run to run and 0.6-4.3% from day to day. Protein recoveries with this coating ranged from 84% to 97%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Amy M. MacDonald, Charles A. Lucy,