Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1209909 | Journal of Chromatography A | 2006 | 7 Pages |
Abstract
An overview on experimental design in quantification experiments using the internal standard technique is described. Particular emphasis is given to the modelling of the relationship analyte/internal standard and the estimation of the optimal amount of internal standard to be used throughout the analytical concentration range by using a uniform shell design with a minimum number of seven experiments. The described approach is demonstrated in the analysis of cholesterol in various reference materials by gas chromatography (GC), using α-5 cholestan as internal standard. The outlined approaches can be applied in the determination of any kind of analyte.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Pedro Araujo, Felicia Couillard, Else Leirnes, Kjersti Ask, Annbjørg Bøkevoll, Livar Frøyland,