Performance of columns packed with the new shell Kinetex-C18 particles in gradient elution chromatography

The performance of columns packed with the new 2.6 μm Kinetex-C18 shell particles was investigated in gradient elution chromatography and compared with those of the 2.7 μm Halo-C18 shell particles and the 1.7 μm BEH-C18 totally porous particles. The peak capacities PcPc of these columns were derived from the resolution of the components of a peptide mixture (ββ-Lactoglobulin digest) and of a mixture of two biomolecules (insulin and lyzozyme).The three columns exhibit the same peak capacities for the peptides at low linear velocity (u0