Article ID Journal Published Year Pages File Type
1210079 Journal of Chromatography A 2006 5 Pages PDF
Abstract

We have developed a miniaturized two-way detection system using thermal lens and fluorescence spectroscopies for microchip chemistry. The system was composed of laser diode (LD) modules, fiber-based optics combined with a gradient index lens, and miniaturized detection units for thermal lens and fluorescence signals. The detection limits in the thermal lens and fluorescence spectroscopies were 6.3 × 10−9 M for Ni(II) phthalocyanine tetrasulfonic acid and 3.0 × 10−9 M for cy5, respectively. The performance of the system with the miniaturized thermal lens was equivalent to that of a conventional thermal lens microscope. The fluorescence sensitivity was comparable to sensitivities offered by conventional miniaturized systems.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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