Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210079 | Journal of Chromatography A | 2006 | 5 Pages |
Abstract
We have developed a miniaturized two-way detection system using thermal lens and fluorescence spectroscopies for microchip chemistry. The system was composed of laser diode (LD) modules, fiber-based optics combined with a gradient index lens, and miniaturized detection units for thermal lens and fluorescence signals. The detection limits in the thermal lens and fluorescence spectroscopies were 6.3 × 10−9 M for Ni(II) phthalocyanine tetrasulfonic acid and 3.0 × 10−9 M for cy5, respectively. The performance of the system with the miniaturized thermal lens was equivalent to that of a conventional thermal lens microscope. The fluorescence sensitivity was comparable to sensitivities offered by conventional miniaturized systems.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Masayo Yamauchi, Manabu Tokeshi, Jun Yamaguchi, Takashi Fukuzawa, Akihiko Hattori, Akihide Hibara, Takehiko Kitamori,