Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210224 | Journal of Chromatography A | 2006 | 6 Pages |
Abstract
A high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD) has been developed for the simultaneous determination of seven polyoxypregnane glycosides, tenacissosides A, B, G, H, I and marsdenosides C, G, in the stem of Marsdenia tenacissima, a Chinese herbal medicine. With a C18 analytical column, the analytes were separated efficiently using methanol-water as the mobile phase in a gradient program. The method limits of detection ranged from ca. 0.3 μg for marsdenoside C to ca. 0.5 μg for marsdenoside G and the method limits of quantitation from 1.0 μg for marsdenoside C to 1.7 μg for marsdenoside G, respectively. The intra- and inter-day precisions of the method were evaluated and all were less than 4%. All the recoveries for the spiked analytes exceeded 90%. This method was successfully used to analyze 19 samples of the stem of M. tenacissima.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jun Deng, Fan Shen, Daofeng Chen,