Article ID Journal Published Year Pages File Type
1210225 Journal of Chromatography A 2006 8 Pages PDF
Abstract
The problem of the additional band broadening arising from the trans-column temperature profile under conditions of significant viscous heat dissipation has been extended by including the retention enthalpy effect. This effect can significantly aggravate the trans-column migration velocity profile of retained species as compared to the already known zero-retention enthalpy case. An approximate analytical solution for the accompanying additional band broadening could be established. The solution is valid in nearly all practically relevant cases and shows how the additional band broadening varies as a function of the generated heat, the retention coefficient and the retention enthalpy. Surveying all possible HPLC operating conditions, it was found that the inclusion of the retention enthalpy effect leads to significantly larger viscous heating plate height contributions than those predicted by the zero-retention enthalpy solution (up to a factor of 10 or more).
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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