Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210318 | Journal of Chromatography A | 2006 | 5 Pages |
Abstract
A highly sensitive HPLC with direct fluorescent detection (λex = 235 nm, λem = 355 nm) was developed for Pb2+ and Cd2+ complexes with an aromatic polyaminocarboxylate, 1-(4-aminobenzyl)ethylenediamine-N,N,Nâ²,Nâ²-tetraacetate as a pre-column derivatizing agent. A reversed phase partition column pretreated by a cationic surfactant was employed. Although this ligand forms thermodynamically stable complexes with various metal ions, only peaks of Pb2+ and Cd2+ were detected with the ligand-centered emission in the HPLC due to the emissive activity and kinetic stability in the dissociation reaction. The detection limits obtained were 1.5 Ã 10â8 and 3.3 Ã 10â9 mol lâ1 for Pb2+ and Cd2+, respectively.
Related Topics
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Authors
Shingo Saito, Naoki Danzaka, Suwaru Hoshi,