Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210406 | Journal of Chromatography A | 2006 | 5 Pages |
Abstract
Erythrocytes were fused with liposome for intracellular derivatization of amino acids in cells. The fusion efficiency was evaluated with capillary electrophoresis (CE) and laser-induced fluorescence (LIF) detection. Reagent fluorescein isothiocyanate (FITC) was enveloped in liposomes and introduced into erythrocytes by fusion between liposomes and erythrocytes. The amino acids in the fused cells were derivated by the introduced FITC and the derivated amino acids were extracted for detection by capillary electrophoresis equipped with laser-induced fluorescence detector. The fusion conditions were investigated. It was found that incubation of liposome and erythrocytes in the presence of 13% polyethylene glycol 6000 (PEG 6000) for 15Â min produced the highest fusion efficiency and kept the erythrocytes stability.
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Authors
Hua Zhang, Juan Du, Jie Zhao, Yue-zhong Li,