| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1210460 | Journal of Chromatography A | 2006 | 4 Pages |
Abstract
pH-zone-refining counter-current chromatography was successfully applied to the separation of cichoric acid from Echinacea Purpurea (L.) Moench. A 3.0 g quantity of sample was separated using the following two-phase solvent system: MtBE–CH3CN–water (4:1:5, v/v), 10 mM trifluoroacetic acid in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and negative ion electrospray ionization mass spectrometry. Double separations were performed with the same solvent system yielding 563 mg cichoric acid at 95.6% purity.
Related Topics
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Authors
Xiao Wang, Yanling Geng, Fuwei Li, Qianshan Gao, Xingang Shi,